PhanerochaeteV1, Main, Exploration, bibRecord, 000F33

An improved transformation vector for the lignin-degrading white rot basidiomycete Phanerochaete chrysosporium.

Identifieur interne : 000F33 ( Main/Exploration ); précédent : 000F32; suivant : 000F34

An improved transformation vector for the lignin-degrading white rot basidiomycete Phanerochaete chrysosporium.

Auteurs : T. Randall [États-Unis] ; C A Reddy

Source :

Gene [ 0378-1119 ] ; 1991.

RBID : pubmed:1879693

Descripteurs français

KwdFr :
- Basidiomycota (génétique), Basidiomycota (métabolisme), Escherichia coli (génétique), Gènes fongiques (génétique), Lignine (métabolisme), Peroxidases (génétique), Plasmides (génétique), Résistance à la kanamycine (génétique), Technique de Southern (MeSH), Transformation génétique (génétique), Vecteurs génétiques (génétique).
MESH :
- génétique : Basidiomycota, Escherichia coli, Gènes fongiques, Peroxidases, Plasmides, Résistance à la kanamycine, Transformation génétique, Vecteurs génétiques.
- métabolisme : Basidiomycota, Lignine.
- Technique de Southern.

English descriptors

KwdEn :
- Basidiomycota (genetics), Basidiomycota (metabolism), Blotting, Southern (MeSH), Escherichia coli (genetics), Genes, Fungal (genetics), Genetic Vectors (genetics), Kanamycin Resistance (genetics), Lignin (metabolism), Peroxidases (genetics), Plasmids (genetics), Transformation, Genetic (genetics).
MESH :
- chemical , genetics : Peroxidases.
- chemical , metabolism : Lignin.
- genetics : Basidiomycota, Escherichia coli, Genes, Fungal, Genetic Vectors, Kanamycin Resistance, Plasmids, Transformation, Genetic.
- metabolism : Basidiomycota.
- Blotting, Southern.

Abstract

In this study, a lignin peroxidase-encoding gene (LIP) of Phanerochaete chrysosporium was disrupted by inserting into its coding region the kanamycin-resistance determinant from Tn903. The resulting recombinant plasmid, pUGLG1: kan, was transformed into P. chrysosporium with the expectation that the disrupted gene might replace the homologous LIP gene in the chromosome. However, the results showed that pUGLG1: kan sequences do not integrate into the chromosome; instead, the plasmid is maintained intact in the transformants in an extrachromosomal state. Our data also show that pUGLG1: kan undergoes replication in P. chrysosporium, is maintained as a circular element, is recoverable from meiotic and mitotic progeny, although at a low frequency, and can be recovered intact by Escherichia coli transformation. These results suggest that the GLG1 component of pUGLG1: kan contains as yet unidentified sequences that allow its autonomous replication in P. chrysosporium transformants.

DOI: 10.1016/0378-1119(91)90403-x
PubMed: 1879693

Affiliations:

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Le document en format XML

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<author><name sortKey="Randall, T" sort="Randall, T" uniqKey="Randall T" first="T" last="Randall">T. Randall</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.</nlm:affiliation>
<orgName type="university">Université d'État du Michigan</orgName>
<country>États-Unis</country>
<placeName><settlement type="city">East Lansing</settlement>
<region type="state">Michigan</region>
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<author><name sortKey="Reddy, C A" sort="Reddy, C A" uniqKey="Reddy C" first="C A" last="Reddy">C A Reddy</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">An improved transformation vector for the lignin-degrading white rot basidiomycete Phanerochaete chrysosporium.</title>
<author><name sortKey="Randall, T" sort="Randall, T" uniqKey="Randall T" first="T" last="Randall">T. Randall</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (genetics)</term>
<term>Basidiomycota (metabolism)</term>
<term>Blotting, Southern (MeSH)</term>
<term>Escherichia coli (genetics)</term>
<term>Genes, Fungal (genetics)</term>
<term>Genetic Vectors (genetics)</term>
<term>Kanamycin Resistance (genetics)</term>
<term>Lignin (metabolism)</term>
<term>Peroxidases (genetics)</term>
<term>Plasmids (genetics)</term>
<term>Transformation, Genetic (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Basidiomycota (génétique)</term>
<term>Basidiomycota (métabolisme)</term>
<term>Escherichia coli (génétique)</term>
<term>Gènes fongiques (génétique)</term>
<term>Lignine (métabolisme)</term>
<term>Peroxidases (génétique)</term>
<term>Plasmides (génétique)</term>
<term>Résistance à la kanamycine (génétique)</term>
<term>Technique de Southern (MeSH)</term>
<term>Transformation génétique (génétique)</term>
<term>Vecteurs génétiques (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peroxidases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term>
<term>Escherichia coli</term>
<term>Genes, Fungal</term>
<term>Genetic Vectors</term>
<term>Kanamycin Resistance</term>
<term>Plasmids</term>
<term>Transformation, Genetic</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Basidiomycota</term>
<term>Escherichia coli</term>
<term>Gènes fongiques</term>
<term>Peroxidases</term>
<term>Plasmides</term>
<term>Résistance à la kanamycine</term>
<term>Transformation génétique</term>
<term>Vecteurs génétiques</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Basidiomycota</term>
<term>Lignine</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Blotting, Southern</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Technique de Southern</term>
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<front><div type="abstract" xml:lang="en">In this study, a lignin peroxidase-encoding gene (LIP) of Phanerochaete chrysosporium was disrupted by inserting into its coding region the kanamycin-resistance determinant from Tn903. The resulting recombinant plasmid, pUGLG1: kan, was transformed into P. chrysosporium with the expectation that the disrupted gene might replace the homologous LIP gene in the chromosome. However, the results showed that pUGLG1: kan sequences do not integrate into the chromosome; instead, the plasmid is maintained intact in the transformants in an extrachromosomal state. Our data also show that pUGLG1: kan undergoes replication in P. chrysosporium, is maintained as a circular element, is recoverable from meiotic and mitotic progeny, although at a low frequency, and can be recovered intact by Escherichia coli transformation. These results suggest that the GLG1 component of pUGLG1: kan contains as yet unidentified sequences that allow its autonomous replication in P. chrysosporium transformants.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1879693</PMID>
<DateCompleted><Year>1991</Year>
<Month>10</Month>
<Day>03</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>07</Month>
<Day>07</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0378-1119</ISSN>
<JournalIssue CitedMedium="Print"><Volume>103</Volume>
<Issue>1</Issue>
<PubDate><Year>1991</Year>
<Month>Jul</Month>
<Day>15</Day>
</PubDate>
</JournalIssue>
<Title>Gene</Title>
<ISOAbbreviation>Gene</ISOAbbreviation>
</Journal>
<ArticleTitle>An improved transformation vector for the lignin-degrading white rot basidiomycete Phanerochaete chrysosporium.</ArticleTitle>
<Pagination><MedlinePgn>125-30</MedlinePgn>
</Pagination>
<Abstract><AbstractText>In this study, a lignin peroxidase-encoding gene (LIP) of Phanerochaete chrysosporium was disrupted by inserting into its coding region the kanamycin-resistance determinant from Tn903. The resulting recombinant plasmid, pUGLG1: kan, was transformed into P. chrysosporium with the expectation that the disrupted gene might replace the homologous LIP gene in the chromosome. However, the results showed that pUGLG1: kan sequences do not integrate into the chromosome; instead, the plasmid is maintained intact in the transformants in an extrachromosomal state. Our data also show that pUGLG1: kan undergoes replication in P. chrysosporium, is maintained as a circular element, is recoverable from meiotic and mitotic progeny, although at a low frequency, and can be recovered intact by Escherichia coli transformation. These results suggest that the GLG1 component of pUGLG1: kan contains as yet unidentified sequences that allow its autonomous replication in P. chrysosporium transformants.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Randall</LastName>
<ForeName>T</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Reddy</LastName>
<ForeName>C A</ForeName>
<Initials>CA</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>1-RO1-GM39032-01A1</GrantID>
<Acronym>GM</Acronym>
<Agency>NIGMS NIH HHS</Agency>
<Country>United States</Country>
</Grant>
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<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
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<MedlineJournalInfo><Country>Netherlands</Country>
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<ChemicalList><Chemical><RegistryNumber>9005-53-2</RegistryNumber>
<NameOfSubstance UI="D008031">Lignin</NameOfSubstance>
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<Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber>
<NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance>
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<Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber>
<NameOfSubstance UI="C042858">lignin peroxidase</NameOfSubstance>
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<CitationSubset>IM</CitationSubset>
<GeneSymbolList><GeneSymbol>LIP2</GeneSymbol>
</GeneSymbolList>
<MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
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<MeshHeading><DescriptorName UI="D015139" MajorTopicYN="N">Blotting, Southern</DescriptorName>
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<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
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<MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName>
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<MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName>
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<MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName>
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<tree><noCountry><name sortKey="Reddy, C A" sort="Reddy, C A" uniqKey="Reddy C" first="C A" last="Reddy">C A Reddy</name>
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	Serveur d'exploration sur le phanerochaete
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Serveur d'exploration sur le phanerochaete

An improved transformation vector for the lignin-degrading white rot basidiomycete Phanerochaete chrysosporium.

An improved transformation vector for the lignin-degrading white rot basidiomycete Phanerochaete chrysosporium.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki